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Bone regeneration after implantation with different hydrogels substitute into rat femoral defects in vivo . (A) Coronal, sagittal, and 3D reconstruction of femoral. (B) Quantitative results of BV/TV in the femoral condyle defects based on <t>micro-CT.</t> (C–D) H&E and Masson staining of femoral section at 4 weeks after implantation. (E) Fluorescent microscope observation of a typical image of Calcein and Alizarin red staining. (F) Quantitative analysis of the mineral supposition rate at 4 weeks after implantation. Data are presented as the mean ± SEM; n = 3; ∗significant difference between selected groups, ∗ p < 0.05, ∗∗ p < 0.01 and ∗∗∗ p < 0.001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
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Bone regeneration after implantation with different hydrogels substitute into rat femoral defects in vivo . (A) Coronal, sagittal, and 3D reconstruction of femoral. (B) Quantitative results of BV/TV in the femoral condyle defects based on <t>micro-CT.</t> (C–D) H&E and Masson staining of femoral section at 4 weeks after implantation. (E) Fluorescent microscope observation of a typical image of Calcein and Alizarin red staining. (F) Quantitative analysis of the mineral supposition rate at 4 weeks after implantation. Data are presented as the mean ± SEM; n = 3; ∗significant difference between selected groups, ∗ p < 0.05, ∗∗ p < 0.01 and ∗∗∗ p < 0.001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
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Bone regeneration after implantation with different hydrogels substitute into rat femoral defects in vivo . (A) Coronal, sagittal, and 3D reconstruction of femoral. (B) Quantitative results of BV/TV in the femoral condyle defects based on <t>micro-CT.</t> (C–D) H&E and Masson staining of femoral section at 4 weeks after implantation. (E) Fluorescent microscope observation of a typical image of Calcein and Alizarin red staining. (F) Quantitative analysis of the mineral supposition rate at 4 weeks after implantation. Data are presented as the mean ± SEM; n = 3; ∗significant difference between selected groups, ∗ p < 0.05, ∗∗ p < 0.01 and ∗∗∗ p < 0.001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
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Bone regeneration after implantation with different hydrogels substitute into rat femoral defects in vivo . (A) Coronal, sagittal, and 3D reconstruction of femoral. (B) Quantitative results of BV/TV in the femoral condyle defects based on <t>micro-CT.</t> (C–D) H&E and Masson staining of femoral section at 4 weeks after implantation. (E) Fluorescent microscope observation of a typical image of Calcein and Alizarin red staining. (F) Quantitative analysis of the mineral supposition rate at 4 weeks after implantation. Data are presented as the mean ± SEM; n = 3; ∗significant difference between selected groups, ∗ p < 0.05, ∗∗ p < 0.01 and ∗∗∗ p < 0.001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
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Bone regeneration after implantation with different hydrogels substitute into rat femoral defects in vivo . (A) Coronal, sagittal, and 3D reconstruction of femoral. (B) Quantitative results of BV/TV in the femoral condyle defects based on <t>micro-CT.</t> (C–D) H&E and Masson staining of femoral section at 4 weeks after implantation. (E) Fluorescent microscope observation of a typical image of Calcein and Alizarin red staining. (F) Quantitative analysis of the mineral supposition rate at 4 weeks after implantation. Data are presented as the mean ± SEM; n = 3; ∗significant difference between selected groups, ∗ p < 0.05, ∗∗ p < 0.01 and ∗∗∗ p < 0.001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
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Bone regeneration after implantation with different hydrogels substitute into rat femoral defects in vivo . (A) Coronal, sagittal, and 3D reconstruction of femoral. (B) Quantitative results of BV/TV in the femoral condyle defects based on <t>micro-CT.</t> (C–D) H&E and Masson staining of femoral section at 4 weeks after implantation. (E) Fluorescent microscope observation of a typical image of Calcein and Alizarin red staining. (F) Quantitative analysis of the mineral supposition rate at 4 weeks after implantation. Data are presented as the mean ± SEM; n = 3; ∗significant difference between selected groups, ∗ p < 0.05, ∗∗ p < 0.01 and ∗∗∗ p < 0.001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
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Bone regeneration after implantation with different hydrogels substitute into rat femoral defects in vivo . (A) Coronal, sagittal, and 3D reconstruction of femoral. (B) Quantitative results of BV/TV in the femoral condyle defects based on <t>micro-CT.</t> (C–D) H&E and Masson staining of femoral section at 4 weeks after implantation. (E) Fluorescent microscope observation of a typical image of Calcein and Alizarin red staining. (F) Quantitative analysis of the mineral supposition rate at 4 weeks after implantation. Data are presented as the mean ± SEM; n = 3; ∗significant difference between selected groups, ∗ p < 0.05, ∗∗ p < 0.01 and ∗∗∗ p < 0.001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
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Bone regeneration after implantation with different hydrogels substitute into rat femoral defects in vivo . (A) Coronal, sagittal, and 3D reconstruction of femoral. (B) Quantitative results of BV/TV in the femoral condyle defects based on <t>micro-CT.</t> (C–D) H&E and Masson staining of femoral section at 4 weeks after implantation. (E) Fluorescent microscope observation of a typical image of Calcein and Alizarin red staining. (F) Quantitative analysis of the mineral supposition rate at 4 weeks after implantation. Data are presented as the mean ± SEM; n = 3; ∗significant difference between selected groups, ∗ p < 0.05, ∗∗ p < 0.01 and ∗∗∗ p < 0.001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
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Bone regeneration after implantation with different hydrogels substitute into rat femoral defects in vivo . (A) Coronal, sagittal, and 3D reconstruction of femoral. (B) Quantitative results of BV/TV in the femoral condyle defects based on micro-CT. (C–D) H&E and Masson staining of femoral section at 4 weeks after implantation. (E) Fluorescent microscope observation of a typical image of Calcein and Alizarin red staining. (F) Quantitative analysis of the mineral supposition rate at 4 weeks after implantation. Data are presented as the mean ± SEM; n = 3; ∗significant difference between selected groups, ∗ p < 0.05, ∗∗ p < 0.01 and ∗∗∗ p < 0.001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Journal: Materials Today Bio

Article Title: Ultrasound-activated piezoelectric Silk-PVDF hydrogel reprograms the osteoimmune microenvironment via NRF2 signaling for accelerated bone regeneration

doi: 10.1016/j.mtbio.2026.102779

Figure Lengend Snippet: Bone regeneration after implantation with different hydrogels substitute into rat femoral defects in vivo . (A) Coronal, sagittal, and 3D reconstruction of femoral. (B) Quantitative results of BV/TV in the femoral condyle defects based on micro-CT. (C–D) H&E and Masson staining of femoral section at 4 weeks after implantation. (E) Fluorescent microscope observation of a typical image of Calcein and Alizarin red staining. (F) Quantitative analysis of the mineral supposition rate at 4 weeks after implantation. Data are presented as the mean ± SEM; n = 3; ∗significant difference between selected groups, ∗ p < 0.05, ∗∗ p < 0.01 and ∗∗∗ p < 0.001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Bone regeneration at the defects site were quantitatively assessed using high-resolution micro-CT (Quantum GX2, PerkinElmer, USA).

Techniques: In Vivo, Micro-CT, Staining, Microscopy